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The bone samples were harvested from human femoral heads during orthopaedical procedures of hip joint implantation.
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Patients and methods: Blood samples were collected before and 24 h after cardiac surgery with CPB and bone marrow samples were harvested directly after sternotomy, before initiation of CPB, and at the end of CPB, before sternal closure.
After 72 h, spleen and bone marrow samples were harvested and snap-frozen in O.T.C buffer.
Samples were harvested after 2, 4, 8, and 12 weeks.
After five days, triplicate samples were harvested.
Samples were harvested approximately every four days.
Histologic samples were harvested including graft sites.
Samples were harvested 1, 6, 12, 24 and 48 hat.
Samples were harvested by centrifugation and washed twice in PBS.
Cell samples were harvested at various times during each perturbation.
Tissue samples were harvested immediately.
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