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The frozen bone samples were freeze-dried using a Lyovac lyophilizer GT2e (Steris, Germany) for 24 hours.
Bone marrow samples were frozen in cryomolds and performed on 5-μm sections.
Twenty-four native cancellous bone (NCB) samples were frozen immediately after harvest and maintained at −20°C until use.
The samples were frozen and processed as described previously49.
Nutrient samples were frozen immediately until further laboratory analyses.
All the samples were frozen at −30°C until use.
All samples were frozen at −70 °C for later analysis.
Plasma samples were frozen at −70°C.
Samples were frozen immediately until use.
Samples were frozen at -80°C.
The water samples were frozen.
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