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Contiguous bone samples were analyzed for undecalcified histomorphometry and for mRNA expression.
Bone samples were analyzed to identify the degree of biofilm growth and infiltration, and to investigate variation in microbial communities with different preservational settings.
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The second half of the bone samples was analyzed by static histomorphometry.
The second half of each bone sample was analyzed by microcomputed tomography.
Peripheral blood or bone marrow samples were analyzed by Affymetrix oligonucleotide microarrays [ 15].
To normalize for variance between tissues, bone marrow samples were analyzed for levels of GAPDH Applied Biosystemss, Taqman rodent GAPDH control kit) with manufacturer-supplied primers and probe (139 nM and 200 nM final concentration, respectively) in conjunction with the Verso 1-Step qRT-PCR kit.
The tissue samples were analyzed in a blinded fashion by two experienced bone pathologists (AN and LRB).
The soil samples were analyzed for about 15 components, including concentrations of calcium, phosphate and nitrogen.
Triplicate samples were analyzed.
Calibration verification samples were analyzed prior to analyzing samples.
Samples were analyzed immediately.
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