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Five non-malignant bone samples were analysed individually.
The bone samples were analysed by using a Thermo Finnigan Element2 ICP-SF-MS instrument by using the isotopes Gd (analyte) and Eu (internal standard) in low-resolution mode.
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Protein associations and networks from the list of proteins with significantly changed abundance levels between no bone disease and high bone disease MM patient serum samples were analysed using STRING Search Tooll for the Retrieval of Interacting Genes), a database of known and predicted protein-protein interactions [ 12].
A rough differential count of cells was performed on the bone marrow samples to prove the presence of a sufficient number of bone marrow cells and the samples were analysed for haematological abnormalities.
For this reason, the drug resistance data of bone marrow and peripheral blood samples were analysed all together.
Fasting urine samples were analysed for bone resorption markers; CTx-I (RatLaps; Nordic Bioscience Diagnostics A/S), helical peptide (Metra, Quidel Diagnostics) and free and total PYD and DPD (Metra, Quidel Diagnostics).
Samples were analysed for traces of a substance called TCPY, a breakdown product of chlorpyrifos.
Samples were analysed in duplicates.
All samples were analysed immediately.
Samples were analysed in triplicates.
Samples were analysed in Kingston University.
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