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Primary human osteoblast-like cells were established in culture from trabecular bone samples obtained from waste materials during orthopedic surgery.
Lead concentrations and stable lead isotopic compositions were measured in blood and trabecular bone samples obtained from five patients who underwent total hip or knee joint replacement.
Furthermore, a comparison of the concentration ratios in bone samples obtained from the smokers and nonsmokers allowed an assessment of Cd and Pb stress.
The trabecular bone samples obtained from patients with OA were architecturally distinct, having elevated bone surface density and Tb.N and decreased Tb.Sp compared to the age- and gender-matched controls.
Cells were cultured for up to seven days in α-MEM medium containing 10% FCS and 10 nM 1α,25-dihydroxyvitamin D3 (1,25 D) [ 26] then stained for TRAP using a commercial kit (Sigma) at Day 7. Human primary osteoblasts were cultured from cancellous bone samples obtained from patients undergoing total hip replacement surgery, as described previously [ 16].
Bone histomorphometry was performed on IT trabecular bone samples obtained from 14 out of the 15 OA cases from total hip replacement (tissue sample size in one case was insufficient) and the 13 controls without evidence of OA pathology taken at autopsy.
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Thirty-four macroscopically normal human cartilage-bone samples obtained from total joint replacements were subjected to standardized single impacts in vitro (range: 0.25 J to 0.98 J).
hOBs were isolated from bone samples obtained under informed consent from female patients undergoing hip or knee replacement surgery (Queensland University of Technology Research Ethics approval number 0600000232) and cultured as described previously (Reichert et al., 2010).
An immunohistochemistry study on normal prostate tissues, primary tumors, and bone metastasis samples obtained from patients revealed that CCN3 expression levels were higher in patients with bone metastasis and positively correlated with malignancy in human prostate cancer cells.
Pirh2 mRNA expression was also determined in bone marrow samples obtained from patients with MM.
VENTX2 antisera detected a 28-kDa protein in cells transfected with a VENTX2 expression construct, in a human erythroleukemic cell line and in bone marrow samples obtained from patients in recovery phase after chemotherapy.
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