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Primary human osteoblast-like cells were established in culture from trabecular bone samples obtained from waste materials during orthopedic surgery.
Hence, our study is based on human bone samples obtained with minimal laboratory manipulation.
Lead concentrations and stable lead isotopic compositions were measured in blood and trabecular bone samples obtained from five patients who underwent total hip or knee joint replacement.
Furthermore, a comparison of the concentration ratios in bone samples obtained from the smokers and nonsmokers allowed an assessment of Cd and Pb stress.
The trabecular bone samples obtained from patients with OA were architecturally distinct, having elevated bone surface density and Tb.N and decreased Tb.Sp compared to the age- and gender-matched controls.
hOBs were isolated from bone samples obtained under informed consent from female patients undergoing hip or knee replacement surgery (Queensland University of Technology Research Ethics approval number 0600000232) and cultured as described previously (Reichert et al., 2010).
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Thirty-four macroscopically normal human cartilage-bone samples obtained from total joint replacements were subjected to standardized single impacts in vitro (range: 0.25 J to 0.98 J).
Bone marrow mononuclear cells (BMMC) were isolated from RA bone marrow samples obtained during joint replacement surgery.
An immunohistochemistry study on normal prostate tissues, primary tumors, and bone metastasis samples obtained from patients revealed that CCN3 expression levels were higher in patients with bone metastasis and positively correlated with malignancy in human prostate cancer cells.
VENTX2 antisera detected a 28-kDa protein in cells transfected with a VENTX2 expression construct, in a human erythroleukemic cell line and in bone marrow samples obtained from patients in recovery phase after chemotherapy.
Pirh2 mRNA expression was also determined in bone marrow samples obtained from patients with MM.
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