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We observed similar effects in a preventive model for breast cancer bone metastasis (data not shown).
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In RT PCR using human-specific primers for RANKL and MMP-13, we detected no expression of RANKL and MMP-13 mRNAs not only in MDA cells cultured in vitro but also in bone with severe metastasis (data not shown).
Bone metastasis is not so infrequent in patients with LACC.
The role of Hsp27 in breast cancer metastases, particularly in bone metastasis, is not clearly elucidated.
Specificity was the proportion of individuals for whom the diagnosis of bone metastasis was not recorded in the chart and who did not have the outcome of interest recorded in structured EHR or claims data.
Expression analysis of these genes in prostate cancer specimens showed CA1 to be highly expressed in bone metastasis but not expressed in primary tumor and EPHA7 to be expressed in normal prostate and primary tumor but not bone metastasis.
As mentioned above, the SBC-3 cell line was established from a bone metastasis and not from a primary site.
In our series, bone metastasis was not detected in 23 stage I patients, indicating that bone scan could be omitted in stage I patients in the future.
The benefit came entirely from delaying bone metastasis, not death.
Metastasis to bone was not detected (data not shown).
Thus, esterified BP7033Br ALK abrogated angiogenesis, both soft tissue and bone metastasis whereas BP7033Br did not.
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