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Adjacency of the site of inflammation to the analysed tibia did not have any influence on bone mass (data not shown).
SRT2104-treated mice subjected to hindlimiceusubjectedhad higher toabecular bone volume, trabecular connectivity, and trabecular bone mineral density, but not cortical bone mass (data not shindlimbompared with the suspensiontrol mice (Fig. S4C).
However, body weight was significantly higher in ARKO mice after 4 (+11%), 6, and 8 (+18%) weeks on an HFD (age 12, 14, and 16 weeks; Fig. 2 A ). Dual energy X-ray absorptiometry confirmed an increase in fat mass (+101%; Fig. 2 C ) and body fat percentage (+62%; Fig. 2 D ) without any changes in absolute bone mass (data not shown) or lean body mass (Fig. 2 B ) in HFD-fed ARKO mice.
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At birth, there were no significant associations between placental amino acid transporter gene expression and neonatal lean mass, fat mass, or bone mineral content (data not shown).
Most exercise programs result in modest bone density gains, about 1%2%%, in premenopausal women; in postmenopausal women, bone benefits are generally measured in bone mass preserved, not gained.
The restoration of bone mass did not recover the original bone strength.
Bone mass also decreased slightly initially and then stayed constant, which makes me think the first reading was a little high: bone mass should not change within days.
Six months after surgery, bone mass had not changed significantly from baseline.
However, the influence of this gut-bone axis on bone mass could not explain the increase in bone mass observed in another study with SSRI's [ 14].
However, bone mass has not been directly measured in these mice.
Peak bone mass may not have been acquired in all participants [ 22].
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