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Human osteoprogenitors from bone marrow were selected using the antibody STRO-1 utilising a magnetically activated cell separation system.
Regions of interest (ROIs) corresponding to MCs were selected manually on the unenhanced image; control ROIs in the "healthy" bone marrow were selected.
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The lineage-negative bone marrow were transduced with retrovirus and selected with puromycin (1 μg/ml) for 1 day after infection.
MSCs from human bone marrow were cultured and magnetically labelled.
In 14 patients, bone marrow was harvested from iliac crest and BM-MNCs were selected by Ficoll gradient.
Bone marrow was harvested from the iliac crest and BM-MNCs were selected by Ficoll gradient.
Bone marrow was obtained at the end of the experiments from a number of randomly selected mice and used to determine the in vitro sensitivity of the grafted bone marrow using the in vitro bone marrow toxicity test.
The bone marrow is clear.
Donating bone marrow isn't a trivial matter.
A three-material decomposition (bone, red bone marrow, yellow bone marrow) was performed for each voxel.
Bone marrow was removed with saline lavage.
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