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Differentiated epithelial cells isolated from the patient and chondrocytes derived from mesenchymal stem cells collected from the patient's bone marrow were seeded onto the scaffold.
Subsequently, whole bone marrow of the bilateral tibia was collected and cells from the whole bone marrow were seeded in 75 cm petri dish at a concentration of 1 × 10 cells/mL.
For immunofluorescence, freshly isolated PMN from the bone marrow were seeded on poly-D-lysin coated coverslips, allowed to adhere, and stimulated with 50 nM PMA for three hours at 37°C.
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The same numbers MSCs isolated from bone marrow were then seeded for a CFU-F assay.
Rabbit bone marrow cells were seeded onto ivory discs as described above and cultured with α-MEM containing 10% (vol/vol) FCS with 100 μM CLO or ALN in the absence or presence of 100 ng/mL recombinant human RANKL (four wells per treatment).
To assess osteoclast activity, bone marrow cells were seeded on 650-μm-thick bovine cortical bone slices (10 cells/slice) in αMEM supplemented with 5 % FBS, 20 ng/ml RANK-L and M-CSF (30 ng/ml).
Mouse bone marrow cells were seeded in 24-well plates containing sonicated cortical bovine bone slices (0.125 0.5 cm) at a concentration of 1 × 106 cells/well.
Canine bone marrow cells were seeded on the scaffolds and cultured in a bioreactor for 3 days.
Subcultured rat bone marrow cells were seeded on the substrates and after 7 days of culture, the implants were subcutaneously implanted in nude mice for 4 weeks.
Rat bone marrow cells were seeded on these scaffolds and chondrogenically differentiated in vitro for 4 weeks followed by subcutaneous implantation in vivo for 8 weeks.
Isolated bone marrow cells were seeded onto 10-ml tissue culture dishes (Thermo Scientific, Tokyo), and cultured with αMEM supplemented with 10% FBS.
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