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To address this question, total levels of CXCL12 present in the bone marrow were quantified by ELISA (Figure 3A).
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In the present study, the level of EPCs in peripheral circulation and in the bone marrow was directly quantified by flow cytometry measurement of the percentage of CD133/KDR double-positive mononuclear cells.
The relative amount of edema, calculated as the percentage of the total bone marrow area, was quantified as described in an earlier study in which bone marrow edema was assessed for a comparative analysis of magnetic resonance (MR) images and histopathology in osteoarthritis [ 15].
Bone marrow cell populations were quantified and phenotyped using a CyAn ADP flow cytometer (Dako, Ely, UK).
The bone marrow is clear.
Donating bone marrow isn't a trivial matter.
A three-material decomposition (bone, red bone marrow, yellow bone marrow) was performed for each voxel.
Bone marrow was removed with saline lavage.
Both bone cells are the bone marrow are affected.
Bone marrow was obtained from the patients.
Bone marrow was isolated from femurs.
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