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Bone marrow was removed with saline lavage.
To isolate RNA from whole bones or whole OS, bone marrow was removed and bones were mechanically crushed with a mortar and pestle and RNA extracted using TRIzol reagent (Invitrogen, Carlsbad, CA) according to standard protocol.
Bone marrow was removed from trabecular bone by the routine technique used in our bone bank.
Using a syringe, bone marrow was removed and resuspended in 10 ml serum-free L-DMEM medium containing 100 IU/ml penicillin and 100 μg/ml streptomycin.
Bone marrow was removed from the femurs with 5 mL of hypotonic solution (0.075 M potassium chloride - Labsinth, São Paulo, Brazil).
Next, bone marrow was removed from all the FDs, which then were first soaked with ether (to remove fat content) and later in H2O2 (to remove remaining blood deposits).
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Mice were injected with 2 mg BrdU i.p. 16 h later, and spleens and bone marrow were removed and made into single-cell suspensions.
The mice were monitored for changes in behavior and body weight for 1 month, after which they were killed; their heart, brain, liver, kidneys, bladder, and bone marrow were removed, fixed in buffered formalin, sectioned, stained with hematoxylin and eosin (H&E), and analyzed histologically.
The bone marrow was then removed via a 10 sec pulse of centrifugation at 2000 rpm.
After the tips of each bone were removed, bone marrow was collected by flushing out the content of femurs and tibias with phosphate buffered saline (PBS).
Femurs, tibiae, and humeri were removed, and bone marrow was harvested by flushing with HBSS (Hanks' balanced salt solution; without Ca2+/Mg2+), 30 mM Hepes, and 15 mM EDTA.
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