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Ordnance gelatin, as substitute bone marrow, was injected into the bone's hollow core.
When a 1∶1 mix of WT and BCS bone marrow was injected into irradiated mice, we observed that about 40% of the thymic developing T cells were of BCS origin and in the spleen the proportion was still about 25%.
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The researchers are now testing how effective the treatment is when the cytokines that mobilize the stem cells from the bone marrow are injected after a mouse heart attack, not before.
Mononuclear cells obtained from MM patient's bone marrow were injected via tail vein in an NRG/SCID mouse.
Donor cells with the competitive cells from Ly5.2 mice bone marrow were injected together into the Ly5.2 recipients in a 4 1 ratio.
The harvested GFP bone marrow was then injected into the retro-orbital plexus of into the irradiated recipients in 100 μL 0.9% saline solution.
In other studies, mice whose bone marrow was destroyed with chemotherapeutic agents were injected with peripheral blood from transgenic animals with germline cells expressing green fluorescent protein.
Concentrated, autologous bone marrow aspirate was injected percutaneously into noninfected, atrophic nonunions of the tibia.
A mixture of 5 × 10 Ighb CD1d +/+ and 5 × 10 Igha CD1d −/− bone marrow cells was injected intravenously into 7- to 12-week-old Ighb CD1d +/+ mice (all on a Balb/c background) that had been 900 Rad irradiated with a cesium source (Gammacell 40, Nordion International Inc. Ontario, Canada) 1 day before.
The bone marrow was flushed out by injecting filtered fetal calf serum (FCS) using a syringe.
R26PR Mx1-cre and Mx1-cR26PR Mx1-crere injected with pIpC and bone marrow was harvested 2 weeks later.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com