Bone marrow specimens were obtained from a patient diagnosed with multiple myeloma under a protocol approved by the Seoul National University Hospital Institutional Review Board.
Bone marrow specimens were obtained at the time of diagnosis during routine clinical assessment of 105 pediatric patients with AML, who presented at the Department of Hematology and Oncology, Children's Hospital of Soochow University between 2006 and 2011.
After 14 weeks, bone marrow specimens were obtained from mice, and isolated bone marrow cells were cultured in RPMI-1640 medium (Gibco-BRL, Gaithersburg, MD, USA) supplemented with 10% heat-inactivated fetal bovine serum, penicillin (100 U/mL), and streptomycin (100 g/mL) (GIBCO, Grand Island, NY, USA).
Fresh primary leukemia patient samples and normal bone marrow specimens were acquired after obtaining informed consent, according to institutional guidelines and the declaration of Helsinki.
Frozen aliquots of Ficoll-isolated mononuclear cells from pretreatment ('diagnostic') peripheral blood or bone marrow specimens from adult patients with AML were obtained from repositories at the Fred Hutchinson Cancer Research Center.
In the absence of difficult-to-obtain bone marrow specimens, microbiologic culture of a blood sample is considered to be the current state-of-the art test for the diagnosis of typhoid fever even though its sensitivity may be as low as 40% [ 4, 5].
After patient informed consent was obtained, peripheral blood and bone marrow specimens were collected according to the Institutional Review Board guidelines of the Mount Sinai School of Medicine.
This was confirmed on evaluation of bone marrow biopsy specimens obtained at the platelet nadir, which demonstrated ample numbers of megakaryoctyes, most of which had actually lost their characteristic appearance, and failed to demonstrate evidence of terminal budding.
Cytogenetic analysis was performed on metaphase spreads obtained from tumor biopsy or bone marrow specimens and cell lines.
KC coordinated and carried out the protocols for obtaining normal peripheral blood and normal bone marrow specimens.
