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ImDCs were generated from bone marrow progenitors using granulocyte macrophage colony-stimulating factor (GM-CSF) [46].
In some experiments, peptide-pulsed bone marrow-derived DC were injected s.c. For immunization with H19-Env peptide, DC were grown from bone marrow progenitors using GM-CSF, following a protocol based on that of Inaba et al. [ 47].
We agree, and in fact we had attempted to knock-down Ankle2 by transduction of bone marrow progenitors using two different shRNA constructs (selected as the best of three when tested in a cell line).
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We then restored p15Ink4b expression in vitro in bone marrow progenitors of these mice using the ProteoTuner system and compared the frequency of erythroid and myeloid progenitors in the presence of Cre, to remove the Rb allele, or in the absence of Cre.
Human bone marrow progenitor cell growth using GM-CSF (CFU-GM) has been used as a predictive assay to identify compounds with myelosuppression capacity (Pessina et al, 2001, 2005).
Cell-based therapy using autologous bone marrow progenitors was rapidly translated into the clinic to treat human ischemic heart disease.
We also used the FMH9 line, derived from primary HoxA9/Meis1-transformed bone marrow progenitors, as an AML model.
Using intra-vital microscopy, we found that loss of PECAM-1 on either bone marrow progenitors or vascular cells resulted in the failure of cells to migrate across the bone marrow vasculature leading to their accumulation within the peripheral circulation.
Using intra vital microscopy we have found that PECAM-1 is involved in the migration of bone marrow progenitors from the blood to the bone marrow.
Here, to understand how cDC1 development changes during tumor progression, we investigated cDC bone marrow progenitors.
Kaplan, R. N. et al. VEGFR1-positive haematopoietic bone marrow progenitors initiate the pre-metastatic niche.
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