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In 30 40% of these patients, individual carcinoma cells can already be detected at the time of primary therapy in cytological bone marrow preparations using immunocytochemistry.
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The team presented a new plan: They would begin chemotherapy again in preparation for a second bone-marrow transplant, perhaps using cord blood this time.
Animals were sacrificed by CO2 gas inhalation at ~24 h after the final administration, and bone marrow preparations were made using the method of Schmid [ 8].
Bone marrow preparations from a wild type mouse were used as a negative control and normal human male preparations were used as a positive control.
Bone marrow-mesenchymal progenitor cells (bm-MPCs; isolated from fresh bone marrow preparations; Lonza Inc., Walkersville, MD, USA) were used as controls.
One mL of bone marrow was used for Cytospin preparation and stained for ICC analysis.
Intravital observations of the bone marrow were made using the skull window preparation previously described by Mazo and colleagues[24].
Whole bone marrow cell preparations were also assayed using other chemotactic agents including MCP-1, KC, RANTES and IL-8, but no difference in the migratory capacity of WT and PECAM-1−/− was observed to these chemotactic agents (data not shown).
Bone marrow fibrosis was graded using European consensus guidelines20.
Gene delivery to cartilage defects using coagulated bone marrow aspirate.
A bone marrow concentration system (BMCS), MarrowStim from Biomet, was used to achieve a rapid preparation of a concentrate of nucleated cells from a sample of bone marrow aspirate.
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