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All together, we noted a higher MS-KIF18A mRNA levels in bone marrow of young animals that was decreased with age.
Mesenchymal stromal cells were isolated from bone marrow of young (21 50 years old; 4 samples) and elderly donors (53 85 years old; 4 samples) as described in our previous work [14], [16].
As expected, we observed co-localization of pγH2AX and 53BP1 at the site of DNA damage foci in the nucleus of all the four Chk1+/− erythroid populations, from least differentiated stage I to more differentiated stage IV erythroblasts isolated from the bone marrow of young non-anemic Chk1+/− mice (Figure 5, Table 1).
To investigate this hypothesis, bone marrow-resident monocytes were isolated directly from the bone marrow of young and old mice.
Long‐term eRapa increased B220+ B‐cell prevalence in spleen and bone marrow of young and aged mice (Fig. S9B) without significant change in absolute numbers in spleen.
To investigate this, bone marrow-resident monocytes, which had developed in vivo, were isolated directly from bone marrow of young and old mice.
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This presents a potential difficulty in assessing the bone marrow of younger patients and patients receiving bone marrow-stimulating factors after CR [26].
Prochymal is a preparation of mesenchymal stem cells, which are obtained from the bone marrow of healthy young adults.
Prochymal is a preparation of mesenchymal stem cells, which are obtained from the bone marrow of healthy young adult donors.
The MSC were isolated from bone marrow of a young pig and grown in basal media (BM) containing DMEM+10% fetal bovine serum and antibiotics.
Therefore, the very low levels of aged B cells achieved in the bone marrow of aged B cells (Ly5.1) into Ly5.2 young adults would not be expected to affect B lymphopoiesis.
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