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MSCs were isolated from bone marrow of transgenic Fischer 344 rats expressing the human placental alkaline phosphatase (hPAP) gene.
As we found that the T-cell population in the spleens of HMGA2 transgenic mice was markedly expanded in comparison with wild-type mice, we decided to also evaluate the T-cell population in the blood and bone marrow of transgenic animals.
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Briefly, the bone marrows of transgenic GFP mice were extracted, disaggregated and cultured in plastic flasks.
In one study, the bone marrow of GFP transgenic mice was transplanted into lethally irradiated syngeneic mice [ 40].
To determine the role of MSCs in liver injury repair, we isolated enhanced green fluorescent protein (EGFP MSCs from bone marrow of EGFP transgenic mice and transplanted them into the mice injected with CCl4 intravenously.
When these API2-MALT1 transgenic mice were crossed with EGFP mice, the deficit of B220+/CD40+ B-cells in the bone marrow of EGFP/API2-MALT1 double transgenic mice (Fig 3C) was restored, further supporting an impact on ubiquitination and NF-κB signalling in vivo.
In order to explore the capacity of myeloid cells to integrate into lymphatic structures in vitro, GFP-labeled macrophages were generated as described above from bone marrow of actin-GFP transgenic mice and subsequently cultured on Matrigel alone or in combination with SV40 T antigen-immortalized murine lymphatic endothelial cells (SV-LEC) [38].
19 Heparanase-specific CTL were generated using peptide-pulsed DC from HLA-A2-positive PBMC and DC from the bone marrow of C57BL/6-Tg transgenic mice.
Dendritic cells were generated from bone marrow of BALB/c and HLA-DR transgenic mice according to published protocols [41] and cultured in the presence of 10 ng/ml GM-CSF.
When the bone marrow of green fluorescence protein (GFP) transgenic donor mice was transplanted into lethally irradiated recipient mice, it was shown that normal FLS contained a minor fraction (1.2%) of GFP+ cells [ 24].
(I and J ) Relative enrichment of Cxcl12 (I) and Nestin (J) mRNA expression in populations sorted from the bone marrow of P7 Nes - Gfp ; Wnt1-Cre2 R26-Tomato Wnt1-Cre2 R26-Tomato Wnt1-Cre2 R26-Tomato
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