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EPCs were isolated from the bone marrow of one day old chickens and cultured in vitro.
(A ) Analysis of the relative abundance of shRNAs M1 to M5 in the peripheral blood of seven mice and the bone marrow of one mouse 12 weeks after reconstitution.
MPDs are clonal haematopoietic stem cell disorders characterized by proliferation in the bone marrow of one or more cell lines resulting in an excessive production of cells belonging to the granulocytic, erythroid and megakaryocytic lineage (Table 1).
Proviral integration was only seen in the wild type mice and in the bone marrow of one of the vector only mice (data not shown).
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As expected, neural crest-traced Schwann cells expressing glial fibrillary acidic protein (GFAP) were also detected in the bone marrow of one-week old mice.
Cytokeratin-expressing cells can be detected in the bone marrow of around one-third of patients with breast cancer and are an independent predictor of poor outcome [ 74, 75].
Isolation of granulomas from the spleens, lungs, and bone marrow of mice after 20 days, one month, and two months following infection was performed as previously described [ 9].
In one study, the bone marrow of GFP transgenic mice was transplanted into lethally irradiated syngeneic mice [ 40].
Human mesenchymal stem cells (hMSC) (Cambrex, East Rutherford, USA), which were harvested and purified from bone marrow aspirates of one healthy donor were purchased (Lonza, Basel, Switzerland) and cultivated in mesenchymal stem cell growth medium (MSCGM) (Lonza, Basel, Switzerland).
Normal bone marrow scintigraphy shows homogeneous activity in the bone marrow of the axial skeleton with uptake in the humeri and femora limited to the proximal one-third.
Perhaps the best example comes from efforts to produce functional blood cells from human iPSCs, where there has been little success in generating a cell type that will engraft into the bone marrow of irradiated mice one of the features of mature blood cells (29).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com