The present study was designed to assess the numbers/production of NK cells in the spleen and bone marrow of aging, normal mice, after in vivo dietary administration of E. purpurea (14 days), or, after injection of thyroxin, a stimulant of NK cell function (10 days).
In order to provide evidence supporting the best source of MSCs for transplantation, we compared the characteristic of cells obtained from the bone marrow of age-matched healthy animals with that of diabetic mice.
Moreover, we assessed the extent of co-localization between γH2AX-foci and telomeres in CD34+ and CD34− cells obtained from cord blood and bone marrow of aged donors.
Consistent with an immature phenotype, total NK cells and NK cell subsets from the bone marrow of aged mice expressed higher frequency of CXCR3.
Even with this major difference in design, our results with primary bone marrow adipocytes directly isolated from the bone marrow of aging mice agree remarkably well with their results.
We have previously reported a decreased number of total NK cells in the blood and spleen and reduced frequencies of CD27− CD11b+ mature NK cells in the blood, spleen, lymph nodes, and bone marrow of aged mice.
We wondered whether there is a similar increase of CD137+ CD4+ T cells in the bone marrow of aged CD137−/− and CD137L−/− mice, and whether the missing increase in myelopoiesis in these mice is due to a deficiency in the CD137+ CD4+ T cells or in the hematopoietic progenitor cells.
As previously indicated (Sherwood et al., 1998, 2000; Alter-Wolf et al., 2009), and shown in Fig. 1B, the remaining pro-B cells in the bone marrow of aged BALB/c mice are low in λ5 SLC and this is also observed for aged B6 mice (data not shown).
Here we report on changes in global gene expression of cultured MSCs isolated from the bone marrow of mice at ages 2, 8, and 26-months.
45 days later, the frequency of mature NK cells (CD27−CD11b+) among total NK cells was measured in the bone marrow of young or aged recipients.
Human MSCs were isolated from bone marrow of 12 donors (mean age 33 ± 21.6 years, 6 female, 6 male).
