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Markers of bone and cartilage turnover, as well as bone marrow lymphocyte phenotypes, indicate different mechanisms for bone loss induced by estrogen deficiency and inflammation, respectively.
Markers of bone remodeling and bone marrow lymphocyte phenotypes indicate different mechanisms for the development of osteoporosis caused by ovariectomy and arthritis in this model.
Furthermore an inverse correlation was demonstrated for BLyS values above median and bone marrow lymphocyte infiltration greater than 50% (P = 0.031, chi square) while sTACI concentrations correlated with b2-microglobulin (P = 0.005), inversely with anemia (P = 0.03), thrombocytopenia (P = 0.04), Binet stage (P = 0.02), and free light chains ratio (P = 0.0003).
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Exley MA, Tahir SMA, Cheng O, Shaulov A, Joyce R, Avigan D et al. Cutting edge: a major fraction of human bone marrow lymphocytes are Th2-like CD1d-reactive T cells that can suppress mixed lymphocyte responses.
The selected control/calibrator was the bone marrow lymphocytes from a healthy donor that was considered as 100% of hsa-miR-34b or -34c expression.
Metaphase spreads were prepared from either cultured MEF cells or bone marrow lymphocytes.
Flow cytometry analysis was performed in order to investigate the effects of treatment on bone marrow lymphocytes.
To confirm the single integration event, an eIF-5A2 probe was used to hybridize to the metaphase spreads from bone marrow lymphocytes by fluorescence in situ hybridization (FISH).
Flow cytometry analysis was performed to evaluate the effects of OVX and arthritis on phenotypes of bone marrow lymphocytes (Table 2).
Flow cytometric examination of transgenic and wild-type bone marrow revealed that 82.7±10.3% (n=5) of bone marrow lymphocytes were T cells versus 9.1±2.5% (n=5, P<0.0001) of those cells in age-matched wild type littermates.
In the mouse, bone marrow lymphocytes commit to the B-cell lineage at the pro-B stage of development (defined CD19+c-Kit+) with V H DJDJ H recombination of the immunoglobulin heavy chain also occurring at this developmental stage.
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