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The bone marrow isolation from donor mice was performed under sterile conditions.
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Moreover bone marrow isolation is a painful and invasive procedure.
BM isolation: Bone marrow cells were isolated from wild type, or CreLyz:Hmox1 flfl, Hmox1 flfl mice and differentiated toward macrophages in RPMI medium (Gibco, Life Technologies, Grand Island, NY, USA) supplemented with 1 20 ng/ml MCSF and 15% fetal bovine serum (FBS) and antibiotics.
Cell culture of splenocytes, purified bone marrow neutrophils (isolation kit Miltenyi) and bone marrow-derived macrophages (BMDMs) were tested.
(B ) Scheme showing FACS isolation of bone marrow stromal populations from Nes-Gfp mice and MSC assays.
The isolation protocol for rat bone marrow from Ahmed et al. [ 15] was modified.
Bone marrow was obtained from the patients.
Multipotent mesenchymal stromal cells (MSCs) raise great interest for brain cell therapy due to their ease of isolation from bone marrow, their immunomodulatory and tissue repair capacities, their ability to differentiate into neuronal-like cells and to secrete a variety of growth factors and chemokines.
After isolation from the bone marrow of BALB/C mice, BMSSCs were expanded in vitro and induced to chondrogenesis or osteogenesis for 14 days, respectively.
No invasive needle extracts the marrow from the bone, and so no "organ" -- bone marrow -- is extracted from the body.
Isolation and culture of bone marrow-derived MSC from paediatric patients with haematologic disorders were performed as described previously [ 19].
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