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However, these changes of a decrease in the erythroid cell population and an increase of erythroid apoptosis were not observed in the bone marrow (data not shown).
Insulin-positive cells did not appear in other organs, such as liver, spleen, lung, and bone marrow (data not shown).
In contrast, inspection of 12-month-old control mice (n = 2) showed metaphyseal regions full of bone marrow (data not shown).
Consistent with the elevated serum anti-HEL Ab levels, increased numbers of anti-HEL Ab-producing cells were detected in the spleens (Figure 1B) and bone marrow (data not shown) of NZB dTg mice.
Earlier attempts using K562eGFPluc cells resulted in an unsatisfactory pathology, with inconsistent tumour cell engraftment of other anatomical locations and not bone marrow (data not shown), similar to a recently published xenograft model using MM1 leukemic cells [46].
We also observed that the efficacy of Jak2i's in such JAK2V617F-driven PV in mice is disproportionately associated with reduced disease burden entirely in spleen, with minimal effect in bone marrow (data not shown).
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Furthermore,I-MIBG therapy early in induction did not affect the yield of stem cell or bone marrow harvest (data not shown).
The MM mSMART stratification for FISH did not predict for high NT-proBNP, nor did it predict for high FLC, or bone marrow plasmacytosis (data not shown).
PCR detection of the TEL-JAK2 transgene and the wild-type Relb allele in thymocytes from these mice showed effective donor bone marrow engraftment (data not shown).
The mild anemia seen in the mutant was not explained by decreased formation of erythrocytes, since the reticulocyte number was 2.5-fold higher in the PiT1neo/neo mice (Table S4), consistent with an absence of abnormalities in bone marrow smears (data not shown).
Kappa statistics ranged from 0.79 to 0.89 for the meniscal changes and 0.78 to 0.87 for the bone marrow edema (data not shown).
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