Sentence examples for bone marrow cells using from inspiring English sources

Relative levels of cytoplasmic aldehyde dehydrogenase (ALDH) were determined in selected subpopulations of normal human bone marrow cells using a flow cytometric assay that simultaneously detects a cell surface antigen (as a marker of cell lineage and developmental stage) and the level of ALDH.

Mononuclear cells were isolated from bone marrow cells using Lymphocyte Separation Medium (Mediatech, Manassas, VA, USA).

Lineage negative (Lin−) cells were enriched from bone marrow cells using the Lineage Cell Depletion kit (Miltenyi Biotec, Auburn, CA) and stained with anti-Sca-1 and c-kit antibodies (BD Biosciences, San Jose, CA).

To define early events in the pathogenesis of this murine model of SA, we compared erythroid differentiation of Sod2-/ and normal bone marrow cells using flow cytometry and gene expression profiling of erythroblasts.

Monocytes/macrophages were isolated from bone marrow cells using a monocyte enrichment kit (StemCell Technologies).

Total RNA was isolated from EML, EPRO and isolated bone marrow cells using trizol reagent (Life Technologies, Gaithersberg, MD).

They include Hare's favored mesenchymal stem cells, which can become bone, muscle, and more; a cocktail of bone marrow cells used in German trials; and bone marrow cells that express a certain marker on their surface called CD34.

As the tumor microenvironment is thought to play a strong role in driving the progression of WM, we also investigated whether the five patient-matched CD19-CD138 bone marrow cells used as controls in this study were defined by any mutations not present in a cohort of more than 1000 controls previously sequenced by Mayo Clinic for genetic studies of other disease states.

c-kit+ cells (1 × 10 cells), selected from murine total bone-marrow cells using CD117 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany), were incubated with the retrovirus and RetroNectin (Takara Bio, Madison, WI, USA) for 24 h in StemPro-34 SFM medium (Invitrogen) containing cytokines (20 ng/mL SCF, 10 ng/mL IL-6 and 10 ng/mL IL-3).

The ends of femur and tibia derived from female C57BL/6 mice (Taconic) were cut and the bone marrow cells flushed using a syringe equipped with a 23-gauge needle.

Fresh or frozen bone marrow cells were used to generate BMDM as previously described [8], using L929-cell conditioned medium (LCCM) as a source of granulocyte/macrophage colony stimulating factor [9].