Control mice received bone marrow cells only.
Control mice received rescue bone marrow cells only.
In bone marrow cells, only the Mac1+ population expressed low levels of MS4a4B, suggesting MS4a4B expression in early hematopoietic progenitors (supplementary Fig. S1A).
Rearrangement of the c-myc oncogene was found in the bone marrow cells only in 5T2 MM transplantation line in a mouse of the 24th generation and in none of the seven other MM of the 5T series which were of earlier generations.
But giving bone marrow cells involves only minimal discomfort -- whether by having a small amount of bone marrow withdrawn from your hip bone under general anesthesia or by having blood taken from veins in your arms after you take 3-4 days of injections to bring cells from your bone marrow into your blood.
In the body, each type seems dedicated to replenishing its own tissue: neural stem cells make new brain cells, bone marrow cells make only red and white blood cells.
Moreover, Miller et al. (2003) showed that all long-term repopulating cells were found in the c-kit+/Sca-1+/Lin (KSL) FGFR1+ fraction of bone marrow cells, although only 0.2% of these were of hematopoietic origin [ 89].
Typically, researchers attempting to further refine gates sort independent populations of cells expressing high, low, or no levels of markers, which makes large numbers of tests difficult when dealing with rare populations (e.g., HSCs represent approximately 0.004% of mouse bone marrow cells, and only 800 1,200 are obtained from a typical mouse bone marrow) [4,14].
The parameters monitored were: body weight, food and water consumption (drinking water study only), hematology, clinical chemistries, cytogentics of bone marrow cells (inhalation study only), mortality, terminal organ weights, and gross and histopathology.
The levels of Slfn4 mRNA were already high in bone marrow cells, and were only moderately elevated in bone marrow from MacBlue/UAS-Slfn4-V5 mice as compared to littermate controls (UAS-Slfn4-V5 and MacBlue mice) (Figure 4B).
The damage induced by the CHOP-like chemotherapy regimen appears to be small enough to render the feedback from bone marrow cells to stem cells not only unnecessary, but even distorting.
