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CD137 protein induced neither cell death in granulocytes among bone marrow cells (not shown) nor in purified granulocytes over a 24 h period (Figure 2).
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Elkabets and colleagues [ 5] determined that the myofibroblasts are not bone marrow derived; thus, the bone marrow cells are not differentiating into myofibroblasts but rather activating the myofibroblasts within the tumor microenvironment.
The analysis of the cytoplasmic mRNA from bone marrow cells was not successful, because cytoplasmic mRNA from bone marrow cells was not stable enough for microarray analysis.
But Hescheler says his recent work proves that bone marrow cells will not differentiate into cardiac cells after transplantation.
However, although BMM incorporated oxidized LDL to give rise to foam cells, the -DHMEQ-treated bone marrow cells did not take up oxidized LDL.
It is also interesting that retrovirus-mediated expression of Hes1 in bone marrow cells does not promote tumorigenesis in transplant recipients [18], [19].
The opposing effects of G-CSF and CD137 protein in reducing or enhancing the macrophage/granulocyte ratio among total bone marrow cells was not observed for hematopoietic progenitor cells indicating that the increased number of monocytic cells among total bone marrow cells resulted mainly from promoting survival of mature granulocytes and macrophages, respectively.
In our experimental setup histamine and CB, which is also a H3R antagonist, target the H4R specifically, since 1) bone marrow cells do not express the H3R [5], 2) cell cycle progression was restored when the H4R was blocked by selective H4R antagonists before exposure to CB or histamine and 3) the effect of CB was clearly reduced after H4R silencing.
Bone marrow cells were not fixed and analyses were done on the same day.
In line with this, Lsd1-deficient bone marrow cells could not contribute to multilineage hematopoiesis in competitive transplantation assays.
Most bone marrow cells did not stain positively and we were unable to detect any staining among bone-lining cells where osteoblasts localize.
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