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The primary objective of this study was to determine the effects of in vivo scanning at 2-week intervals on bone healing of a critical sized radial defect in rabbits and to investigate the effect of this radiation protocol on bone marrow cell viability using clinically applicable radiation doses.
Although 17-DMAG did not significantly improve the bone marrow cell viability at day 3 compared to irradiated vehicle-treated animals, 17-DMAG did markedly promote bone marrow cell viability at day 7 (17-DMAG/RI vs. vehicle/RI: 6.36 ± 1.75 × 10 vs. 2.0 × 10 ± 2.48 × 10) and day 15 (8.26 ± 1.84 × 10 vs. 3.46 × 10 ± 2.91 × 10) after irradiation.
The consistent results from both methods indicate that 17-DMAG protects bone marrow cells from irradiation, suggesting the improved bone marrow cell viability may contribute to increased 30-day survival in irradiated mice pretreated with 17-DMAG.
After erythrocytes were lysed by red blood cell lysis buffer (BioLegend San Diego, CA, USA), total bone marrow cell viability from each mouse was assayed by trypan blue staining [ 12].
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We used a monoenergetic (70 KeV) X-ray sources that was reported to be safe for both structural bone endpoints and bone marrow cell viabilities in small animals [91].
DLB trained and assisted KRS with cytopreps of bone marrow and evaluated smears for marrow cell viability.
BMC: bone marrow cell.
A total protein content assay was used to evaluate the viability and proliferation of cultured bone marrow cells.
Preliminary research suggests that the insertion of autologous bone marrow cells after myocardial infarction can improve left ventricular function and myocardial viability [ 8].
These positive effects of Ris in bone formation were confirmed by in vitro data as the viability and expression of b-ALP gene in bone marrow cells resulted increased in a dose dependent manner.
The novel cytokine was shown to stimulate the viability of monocytes and colony formation of macrophages from bone marrow cells.
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