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All bone marrow cell recipient mice showed extramedullary hematopoiesis in spleen, but no significant pathological change was observed in other organs.
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(e ) Representative FACS plots of bone marrow cells in recipient mice 7 days post Ddb1 deletion.
The persistence of clonal dominance in recipient mice for more than 6 months after bone marrow cell transplantation, and in recipients of secondary transplants, indicates that the affected multipotent hematopoietic cells have both self-renewal and long-term repopulating ability (Morrison and Weissman, 1994).
These studies support the value of CAFC measurements for predicting the fate and growth of transplanted bone marrow cells in recipients pretreated with a variety of cytotoxic agents.
BMC: bone marrow cell.
Irrespective of the genotype of the transferred bone marrow cells, PI3Kγ−/− recipient mice exhibited similar inflammation at the site of infarction (Figure 3E).
Flow cytometry analysis showed that cells expressing CALM-AF10 and NES2-AF10 in the bone marrow cells of primary recipient mice were Mac1+, CSF1R+ and c-kit+ (Fig. 4a).
(A – C ) Transplantation of Nur77 KO donor bone marrow cells into WT recipients conferred elevated bone resorption compared to WT control donor bone marrow cells.
Bone marrow cells from transplanted C57BL/6 CD45.2 Thy1.2 recipient mice (i.e., primary transplant recipients) were harvested at day +65, and injected intravenously into lethally irradiated C57BL/6 CD45.2 Thy1.2 mice (i.e., secondary transplant recipients) (one to one matched transplant).
Eight hours after the final radiation dose, mice were injected in the tail vein with the donor bone marrow cells (1×106 cells per recipient mouse).
(D – F ) Transplantation of WT donor bone marrow cells into Nur77 KO recipients rescued the bone resorption to a level similar to the WT control recipients.
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