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In this study, the orientation-regulated immobilization of a Notch ligand was designed to achieve the efficient Notch ligand receptor recognition for the ex vivo proliferation of a bone marrow cell population containing HSC.
Thus, protecting the regenerative potential of the bone marrow cell population during the challenge of disuse may be critical for tissue recovery during reambulation.
After testing six different sorted subpopulations of GFP positive bone marrow on GFP negative animals, we found that the bone marrow cell population Sca+/Mac-1− can migrate into islets more efficiently than WBM and the other five cell subpopulations.
Supporting the hypothesis that the ability of bone to recover will correlate with the status of the bone marrow cell population, subsequent experiments showed that vibrations applied during disuse, rather than reambulation, were more effective in altering bone morphology upon reambulation.
The percentages of each bone marrow cell population for each technique were compared using the Wilcoxon test.
In both cases, a bone marrow cell population with a higher density of the cell surface receptor c-kit, showed repopulation of murine cardiac myocytes after experimental myocardial infarction (Jackson et al, 2001; Orlic et al, 2001).
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Bone marrow cell populations were quantified and phenotyped using a CyAn ADP flow cytometer (Dako, Ely, UK).
Failure of the bone structure to recover on reambulation may in part be caused by the collapse of the osteogenic potential of bone marrow cell populations during disuse.
It was previously described that some bone marrow cell populations, including MSC, express neural markers even before any neural induction protocol [3], [38] [42].
Here, we tested whether specific bone marrow cell populations as well as trabecular bone morphology can benefit from the application of low-level whole body vibrations during disuse and reambulation.
It serves as another step towards a comprehensive model of hematopoiesis comprising models of stem cell regulation, lineage commitment, bone marrow cell populations, growth-factors and chemotherapy actions.
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bone marrow cell implantation
bone marrow cell differentiation
bone marrow cell loss
bone marrow cell content
bone marrow cell morphology
bone marrow cell proliferation
bone marrow cell migration
bone marrow cell suspension
bone marrow macrophage population
bone marrow cell therapy
bone marrow cell adhesion
bone marrow cell transplantation
bone marrow cell homeostasis
bone marrow cell mobilization
bone marrow cell invasion
bone marrow cell growth
bone marrow cell expansion
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