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Differential bone marrow cell counts revealed increases in the myeloid:erythroid ratio associated to increased numbers of granulopoietic cells in the different clinical groups compared with non-infected dogs.
Nucleated bone marrow cell counts were also reduced in the WT offspring of P2Y14 dams exposed in utero to IR.
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Fresh bone marrow cells were counted using a hemocytometer, centrifuged for 5 minutes at 200× g at 4°C and gently resuspended to obtain a solution containing from 4 to 6×106 cells/ml (unless otherwise mentioned) in freezing media containing 90% fetal bovine serum and 10% DMSO (unless otherwise mentioned).
On day 7, bone marrow cells were counted with trypan blue exclusion using a hemocytometer to assess survival.
The del 5q) samples had bone marrow myeloblast counts ranging from 0 17%, and an abnormal chromosome 5q31.2 in 5 100% of their bone marrow cell metaphases.
BMC: bone marrow cell.
Zr retention by living radiolabelled eGFP-positive cells in vivo was monitored by FACS sorting of liver, spleen and bone marrow cells followed by gamma counting.
Peritoneal cells and bone marrow cells were harvested, and differential counts were performed by flow cytometry.
The CFU count of bone marrow cells from BALB/C mice was significantly increased after 7, 11, and 14 days of SVPII treatment.
The HCE treatment did not decrease the number of CFU in the peritoneal cavity fluid (Control: 5.6 ± 0.1, HCE 5: 5.5 ± 0.2, HCE 50: 5.5 ± 0.1 Log10) The HCE treatment induced a significant decrease on the bone marrow cells number and in the platelets count in peripheral blood when compared to the control group.
However, when BMMs were counted after treating bone marrow cells with M-CSF, no difference was observed in KO and FL mice (Figure 6C), although OGR1 was also expressed in bone marrow cells and PBL (Figure 2A).
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