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In summary, radiation-induced death of marrow cells is associated with 1) a transient increase in bone formation due, at least in part, to activation of bone lining cells, and 2) an increase in bone resorption due to increased osteoclast perimeter.
However, osteoblast lineage cells that are found within the subendosteal niche include osteoprogenitor cells, quiescent bone lining cells, and active osteoblasts [11], [24].
Some of these cells show processes extending into canaliculi, and gap junctions are also observed between adjacent bone lining cells and between these cells and osteocytes [ 50, 51].
Bone remodeling is a multicellular and multispatial event that takes place with the close cooperation among the cells that constitute the so-called basic multicellular unit (BMU), which includes osteoclasts, osteoblasts, osteocytes embedded in the bone matrix, the bone lining cells, and the capillary blood supply (reviewed in [ 21]).
The ALP expression is associated with formation of osteoprogenitors that proliferate and differentiate into identifiable osteoblasts, bone lining cells, and finally, to a new bone formation [72].
Cells of the osteoblast lineage are derived from mesenchymal stem cells, and are represented in this unit by osteoblasts, bone lining cells and osteocytes.
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The cycle is completed by coordinated actions of osteocytes and bone lining cells [ 10, 11].
Bone remodeling is a highly complex cycle that is achieved by the concerted actions of osteoblasts, osteocytes, osteoclasts, and bone lining cells [ 3].
These cytoplasmic processes are connected to other neighboring osteocytes processes by gap junctions, as well as to cytoplasmic processes of osteoblasts and bone lining cells on the bone surface, facilitating the intercellular transport of small signaling molecules such as prostaglandins and nitric oxide among these cells [ 66].
There was a strong association between radiation-induced marrow cell death and activation of bone lining cells to express the osteoblast phenotype (Pearson correlation − 0.85, p < 0.0001).
stimulate progenitor cell recruitment and activate formerly quiescent bone lining cells.
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