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Bone histomorphometry will be performed in a subgroup of 50 subjects.
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Bone histomorphometry can be used to assess the trabecular structure in animals, but it is invasive, destructive, as well as labor and time intensive.
Bone histomorphometry parameters were then analyzed using BIOQUANT OSTEO software (BIOQUANT Image Analysis Corporation, Nashville, TN, USA).
More bone histomorphometry data are also needed.
Cortical bone histomorphometry data are summarized in Table 2.
The standard nomenclature for bone histomorphometry variables was used [ 15].
Tibia trabecular bone histomorphometry data are summarized in the Table 2.
Validation studies to correlate F-PET with bone histomorphometry have been performed (Messa et al. 1993, Piert et al. 2001).
Bone histomorphometry was conducted using 0.5-N loads, and therefore the strain was also measured with the same loading conditions.
Bone histomorphometry was performed to further characterize the bone phenotype and to find out whether it is a consequence of the cartilage defect or a primary bone defect.
Bone histomorphometry was performed using a semi-automatic image analysis system (Bioquant Image Analysis Corporation, Nashville, TN) linked to a microscope that was equipped with transmitted and fluorescent light.
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