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Immunohistochemistry, micro-CT, and bone histomorphometry were performed on the specimens.
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Bone histomorphometry was performed on the double-fluorescent-labeled proximal tibial metaphysis.
Bone histomorphometry was performed on the proximal metaphysis of tibia and on L2 vertebrae (L2).
Bone histomorphometry was performed on the proximal tibiae and tibial shafts for bone turnover parameters at week 40.
Bone histomorphometry was performed on the triple-labeled undecalcified fourth sagittal (LVL-4) and fifth transverse (LVX-5) sections.
Dynamic bone histomorphometry was performed on 7 µm thick sections of mineralized lumbar vertebrae embedded in methylmethacrylate using standard procedures.
Bone histomorphometry was performed using a semi-automatic image analysis system (Bioquant Image Analysis Corporation, Nashville, TN) linked to a microscope that was equipped with transmitted and fluorescent light.
Bone histomorphometry was performed as described elsewhere [23].
Bone histomorphometry was performed to further characterize the bone phenotype and to find out whether it is a consequence of the cartilage defect or a primary bone defect.
Subchondral bone histomorphometry was performed on three nonconsecutive sections of each specimen using our previously published method [ 18], modified from that of Matsui and coworkers [ 19].
Bone histomorphometry was performed using a microscope (Nikon, Tokyo, Japan) equipped with a video camera and digital analysis system (OsteoMeasure; OsteoMetrics, Decatur, GA, USA).
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