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These positive effects of Ris in bone formation were confirmed by in vitro data as the viability and expression of b-ALP gene in bone marrow cells resulted increased in a dose dependent manner.
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In the histomorphometric result, new bone formation was confirmed.
Moreover, new bone formation was confirmed along the newly formed blood vessel in the MSC-CM group, although newly formed blood vessels in other groups were fewer with less bone formation (Fig. 2c).
Furthermore, bone formation was confirmed in 2 IVDs treated with 250 μg rhBMP-7.
A circadian rhythm in bone formation was confirmed using radiolabeled proline and galactose, which demonstrated that synthesis and secretion of ECM components by hypertrophic chondrocytes and osteoblasts exhibited a ~24 h rhythm (Igarashi et al. 2013).
The positive effect of strontium ranelate on bone formation was confirmed by a higher osteoblastic surface area (Ob.S/BS) in treated compared with untreated patients (+38%; p=0.047) and a greater mineral apposition rate (MAR) in trabecular (+8%; p=0.008) and cortical (+11%; p=0.033) bone.
After 42 days of fracture, bone healing and bridging callus formation was confirmed by X-rays in 16 out of 20 cases (80%) and in 18 out of 20 cases (90%), respectively.
After 28 days of fracture bone healing and bridging callus formation was confirmed by X-rays in 12 out of 20 cases (60%) in the study group (Fig. 1b) and in 16 out of 20 cases (80%) in the control group.
Lipid droplet formation was confirmed by oil red O staining.
Autophagosome formation was confirmed further by measuring LC3 expression.
Micelle formation was confirmed by NMR spectroscopy.
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