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Small spicules of dermal bone may occasionally extrude through the epidermis, although bone formation does not originate in the epidermis.
Sp7, also known as Osterix, is required for osteoblast differentiation since in Sp7 null mice bone formation does not occur [ 7].
70 Radiographic progression (mainly new bone formation) does not seem to be inhibited by anti-TNF therapy, 71 but there is also no evidence that syndesmophyte formation is accelerated.
In human arthritis, areas with inflammation were shown to be predictive of bone formation [ 42], but bone formation does not seem to be blocked by anti-tumor necrosis factor alpha (TNFα) treatment [ 43].
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However, bone formation did not exceed 12% of the implant surface, both for the intramuscular and subcutaneous recipient site.
The changes in the percentage of mineralized surface, another index of bone formation, did not reach statistical significance in any of the groups (Fig. 2l).
Markers of bone formation did not correlate with BMD in our subjects.
Furthermore, the accentuation of the BMP-induced ectopic bone formation did not exist in osteopetrotic c-Fos-deficient mice.
Serum osteocalcin, a marker of bone formation, did not differ between control (29.8 ± 6.9 ng mL−1) and iLID mice (28.1 ± 11.5 ng mL−1) at two years of age.
In c-Fos-deficient mice, which are completely osteoclasts deficiency, the accentuation of the BMP-induced ectopic bone formation did not exist.
In a third donor bone formation did not occur in scaffolds however was observed in chondrogenically primed pellets, which will be discussed further.
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