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To analyze bone expression on the protein level, we took advantage of an antibody against the human KRM2 protein.
The ratio of Opg/RankL that favors reduced osteoclastogenesis, and the bone expression of tartrate-resistant acid phosphatase (Trap), a marker of bone resorption, was reduced in heterozygous Pkd1+/Δ mice (Table 1).
We used western blot to measure osteopontin (OPN) serum levels and immunohistochemical staining to examine bone expression.
In this same direction, an increased bone expression of Scl was found in rodents and patients with CKD [ 8].
Therefore, we assayed for tumor and bone expression of RANKL, both of which are readily distinguished by PCR with species-specific primers.
This showed that TGFbeta1 injection alone increased the cartilage/subchondral bone expression of Acan (2.39-fold) and Col2a1 (5.77-fold), whereas Col10a1 was essentially unaffected (Table 1).
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In bone, expressions of MCSF and DKK1 were shown in stromal cells of the bone marrow, in contact with metastatic foci of both tumours.
In addition, the total amount of RANKL resulting from the sum of cartilage, synovium, and subchondral bone expressions was considered as the RANKL global expression in joint tissues.
The only thing that matches ABC's too-close-to-the-bone expression of a corporately-sponsored Christmas is America's annual Black Friday demolition derby and the desperate consumerism that fuels it.
These cells have been demonstrated to recapitulate critical aspects of osteoclast formation and activity, including bone resorption, expression of calcitonin receptor, multinuclear fusion, and TRAP expression [ 6, 34].
Sclerostin was expressed in osteocytes of the subchondral bone, the expression in joint cartilage restricted to chondrocytes in the calcified zone.
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CEO of Professional Science Editing for Scientists @ prosciediting.com