The OTC bone supplement, Citracal Plus Bone Density Builder, is based on a bone support formula already on the market (Citracal) and uses literature support to justify the addition of genistein [ 11, 12].
The cross-sectional image files of the femur were transferred to a custom-written automatic contouring program (Caotool) for the detection of the contours between the cortical bone and cancellous bone based on a dynamic density-thresholding algorithm.
To assess BMD, second to fourth lumbar spine and from the femur bone (neck, trochanter and the whole femur), bone density was calculated based on gr/cm.
Three different volumes were delimitated from the cortex to the medullar space and a percentage of bone density was calculated based on a densitometric analysis of the white voxels, the cortical bone being taken as a reference (100%).
The bone density was evaluated based on the light microscopic inspection of stained sections, disclosing a distinct difference in bone structure on either side of the epiphyseal tubercle.
However, long-term exercise regimes are known to increase bone mass density and based on this, it seems reasonable to expect enhancement of collagen synthesis as a result of exercise.
