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Van Gieson staining showed that most of bone defects were replaced by the new bones and scaffolds were largely degraded in the group of nHA/CS/PLGA + hUCMSCs.
Azan Mallory staining showed that areas of the bone defects were filled with mature collagenous fibers in the blank, Matrigel™ alone, monolayer MSC/Matrigel™ groups (right column in Figure 9A).
The chin bone and mandibular body bone were harvested when the bone defects were present in those areas.
After complete wound healing, the bone defects were managed.
Bone defects were an isolated finding 28.8% of the time.
On each side, 2 bone defects were drilled into the parietal bone and 1 into the frontal bone.
Large bone defects are a considerable challenge to reconstructive surgeons.
Bone defects are usually caused by trauma and nonunion, and autologous bone graft is commonly known as a gold standard in reconstruction of bone defects [ 1].
Strategies for repairing large bone defects are increasingly oriented towards bone graft substitutes and bone-tissue-engineering scaffolds.
Bone sections were stained with toluidine blue stain.
Femoral bone and sternum sections were stained with Giemsa solution.
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