Sentence examples for bone cells using from inspiring English sources
GLP-1R mRNA and protein expression were evaluated in the bone, bone marrow and bone cells using RT-PCR and immunohistochemistry.
On the other hand, we induced the de-differentiation of fully differentiated fat and bone cells using a hypoxia/DHP-d system.
So far, few studies have described the possibility to establish a 3D culture model for bone cells using mineralized porous scaffolds as templates, which relies on the use of a perfusion-based bioreactor device, highlighting the synergism between a bioactive scaffold and the effect of perfusion on cells and indicating the differentiation into an osteogenic phenotype [ 100, 101].
Bone marrow mesenchymal stem cells were obtained by immunomagnetic sorting of mouse bone marrow cells, using antibodies recognizing the specific markers STRO-1 and c-kit (Myilteny Biotech).
For mice, bone marrow‐derived DC (BMDC), a representative of myeloid DC (mDC) and plasmacytoid DC (pDC), are prepared from bone marrow cells using granulocyte/macrophage colony‐stimulating factor (GM‐CSF) or Flt3 ligand, respectively.
Relative levels of cytoplasmic aldehyde dehydrogenase (ALDH) were determined in selected subpopulations of normal human bone marrow cells using a flow cytometric assay that simultaneously detects a cell surface antigen (as a marker of cell lineage and developmental stage) and the level of ALDH.
Mononuclear cells were isolated from bone marrow cells using Lymphocyte Separation Medium (Mediatech, Manassas, VA, USA).
Lineage negative (Lin−) cells were enriched from bone marrow cells using the Lineage Cell Depletion kit (Miltenyi Biotec, Auburn, CA) and stained with anti-Sca-1 and c-kit antibodies (BD Biosciences, San Jose, CA).
To define early events in the pathogenesis of this murine model of SA, we compared erythroid differentiation of Sod2-/ and normal bone marrow cells using flow cytometry and gene expression profiling of erythroblasts.
Monocytes/macrophages were isolated from bone marrow cells using a monocyte enrichment kit (StemCell Technologies).
Total RNA was isolated from EML, EPRO and isolated bone marrow cells using trizol reagent (Life Technologies, Gaithersberg, MD).
