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Isolation and characterization of bone cells from inside bone (osteocytes).
Analysis of the size of bone cells from fossils shows that the saurischian dinosaurs — the branch of dinosaurs from which birds are descended — evolved small genomes (and, presumably, high metabolisms) early on.
Damian Medici, an instructor of medicine at Harvard Medical School and Beth Israel Deaconess Medical Center, found that, unlike normal skeletal tissue, the pathological cartilage and bone cells from these patients contained biomarkers specific for endothelial cells (cells that line the interior of blood vessels).
In addition, since the morphological differences between tumor cells and sloughed cells from the femur is very distinct, we were also able to discern the bone cells from tumor cells.
IMPACT: Combinatorial histomorphometric and functional analyses of bone cells from a mouse model of mucolipidosis II provided novel insight into pathomechanisms and targets for therapeutic intervention which might also be applicable for other lysosomal disorders with skeletal defects.
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For this, she printed finger bones, which she seeded with a type of bone cell from mice (MT3J3 osteoblasts).
For instance, the size of osteocytes — bone cells — can be measured from cross sections of fossil bone.
Inducible genetic fate mapping confirmed that new bone cells do not arise from dedifferentiated osteoblasts under these conditions.
Bone marrow cells from healthy donors were collected during bone marrow aspiration for allogeneic stem cell transplantation.
Bone marrow-derived macrophages were generated by expansion of bone marrow cells from 3 6 month old mice as described previously.
Using mouse models of diabetes, Orlandi et al demonstrated that bone marrow cells from diabetic mice receiving sub-lethal doses of radiation exhibited significantly lower engraftment and repopulation capacity compared to bone marrow cells from healthy mice.
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