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The degrees of bone-implant contact and bone area within the 29 threads measured were 81% and 87%, respectively.
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The amount of new bone was quantified as a percentage of the bone area to the total area within the images using image analysis software, Image J (National Institute of Health, MD, USA) [ 22].
In order to confirm the above results, we also examined the bone area percentage within all threads and the BIC with all threads along the total length of the implant surface.
The number of TRAP-positive multinucleated osteoclasts was quantified within the subchondral bone area comprising the area between the cartilage/bone junction and the growth plate following the standard convention.
The means of the bone area percentage within all threads are shown in Figure 3(a).
This new-formed remodelled bone area includes the new-formed marrow cavity within the graft as well as graft remnants that has been surrounded by new bone.
This new-formed remodeled bone area includes the new-formed marrow cavity within the graft as well as graft remnants that had been surrounded by new bone.
Whereas the bone area density in the control group remained unchanged between days 5 and 10, the bone area density in the test group almost doubled within the same time frame (no statistical difference).
Subsequently, the percentage of bone (bone area %) in the porous area of the specimen was calculated by bone area/porous area.
Then, a region of interest (ROI) of subchondral bone at the level of the enthesis was selected within the volume of the bone area where the PT inserts.
Furthermore, the area of mineralized bone (trabecular bone within the callus area) was determined, but there were no differences within the three groups (Data not shown).
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