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However, 16-week cortical bone, analyzed at the mid-diaphysis, showed significant reductions in Ct.Ar and Ct.Th in iLID mice depleted of serum IGF-1 at 4 weeks of age (Table 1, Figure 4A).
Thus the bone analyzed from individuals in the age range of 60 to 80 years still had measurable amounts of Pb present.
We then calculated the normalized mean volume from three control and six mice with CIA for each bone analyzed (right and left femurs, right and left humeri, and right and left tibias).
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Bones analyzed in this work were previously decalcified with EDTA 0.5 M.
For autosomes, this was 12.7% for each of the three bones analyzed.
But all of the roughly 17,000 animal bones analyzed so far at Dmanisi belong to Eurasian species, not African ones, according to biological anthropologist Martha Tappen of the University of Minnesota in Minneapolis.
For this purpose, 8-week-old KΔ75 and WT female mice were ovariectomized and sacrificed 4 weeks later and their long bones analyzed by histomorphometry (Figure 1A).
Data from scanning elliptical region of interest (ROI, Figure 1B) including only trabecular bone, was analyzed using software CirsCT Bone Densitometry of CIRS company supplied by scanner manufacturer.
Significant force x repetition interaction effects were observed for each bone morphological attribute and serum biomarker of bone turnover analyzed at 12 weeks of task performance.
Forty percent of the total length of each bone was analyzed, including metaphysis and diaphysis regions to verify differences between bone mass of healthy and osteoporotic groups.
Bone structure of the secondary spongiosa of the periarticular and axial bone was analyzed using histomorphometry.
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