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For instance, genetic and bone analyses showed that Windeby Girl, a body discovered in Germany and once deemed female because of its slight frame and long hair, was actually an undernourished male, and the remains are now called Windeby I.
Since the KO mice were markedly smaller in size compared to litter mates and it was previously reported that Wwox KO mice had defects in bone formation [16] we decided to perform bone analyses of our Wwox KO mouse.
Again, 3D and 2D reconstructions did not unveil gross abnormalities of lumbar spine morphology (Figure 4A), but trabecular bone analyses revealed a significantly reduced bone volume per total volume (BV/TV, 18.9 +/− 1.5% vs 15.7 +/− 2.5%) in Lrp4 ECD mutants as compared to wild type littermates, accompanied by the according changes in trabecular separation and trabecular number (Figure 4B).
Mice in both cohorts underwent sham or ovariectomy surgery with bone analyses on the combined cohorts after 6 weeks.
Since these established skeletal phenotypes are unambiguous, no bone analyses were performed on additional cohorts of mice.
For cartilage and bone analyses, a number of embryos were incubated to day 13 in normoxic, 9%, 11%, 13 %, 15 % and 17O2 conditions.
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Pairwise comparison with bones analysed by 3D-QM clearly demonstrated that on average 96.6 ± 1.10% of SECs were not detected using current FC methodologies (Fig. 3h).
To test this hypothesis, we performed bone histomorphometric analyses and microcomputed tomography (micro-CT) analyses of 2-month-old Cthrc1-null and wild-type mice.
We're using 3-D CT scans to perform detailed bone structural analyses of the arm, leg, spine, and hip.
At time of death, femora, L2 vertebrae and tibiae were processed for bone histomorphometric analyses and tibial cortical strength by 3-point mechanical testing.
Five patients had x-rays of their thoracolumbar spine, and 9 patients had bone mineral analyses; all scans were grossly abnormal.
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