Sentence examples for bonds were reduced using from inspiring English sources
The amide bonds were reduced using a 1.0 M borane tethahydrofuran (THF) complex solution (40-fold excess per amide) heated at 65 °C for 72 h followed by overnight treatment with piperidine at 65 °C.
Equal amounts of proteins from both sets were reduced using 5 mM DTT (Sigma, St . Louis MO, USA) at 56 °C for 25 min to reduce disulfide bonds.
The double bond and nitro group of this intermediate is reduced using either catalytic hydrogenation or by treatment with lithium aluminium hydride (method 4).
Protein content was measured using a BCA assay (Thermo Scientific), disulfide bonds were reduced with dithiothreitol (DTT), and cysteine residues were alkylated with iodoacetamide as previously described.
Disulfide bonds were reduced with 5 mM dithiothreitol and cysteines alkylated with 10 mM iodoacetimide.
Disulfide bonds were reduced by DTT additive and finally blocked by treatment with iodoacetamide.
Any potential disulphide bonds were reduced by incubating overnight with 50 mM TCEP at 4 °C.
The disulfide bond is reduced by intracellular reducing molecules (e.g., glutathione) to release the payload.
The double bond was reduced by NaBH4 in the presence of catalytic CoCl2.
Studies indicated that shear bond strengths were reduced when eugenol-based cements were used to lute temporary crowns or inlays [31, 35], although this point has been contested [19].
