Sentence examples for bonding with the active from inspiring English sources
OPs are a class of chemicals that inhibit serine esterases by covalently bonding with the active site serine.
The substitutions V199A and K113N are in close proximity to the CXXC active site, with K113 having direct hydrogen bonding with the active site cysteine 109, known to be involved in deprotonation of the resolving cysteine (31).
In this context, we have developed a catch-and-release reagent that first forms a covalent bond with the active center thiol of a cysteine protease to suppress its activity and then is removed by UV-irradiation to release the parent active protease.
A peptidomimetic inhibitor AG7088 was developed to inhibit the 3C protease of rhinovirus (a member of the family), a chymotrypsin-like protease required for viral replication, by forming a covalent bond with the active site Cys residue.
In the optimized geometries, 7a and 7b, NO2 − is loosely bound in the active site pocket and make hydrogen bonds with the active site residues Asn52 and Gly549.
The presence of mono-valent ions except HCO3 − that is NO3 − and Cl− showed little effect on fluoride adsorption because of the formation of weak bonds with the active sites at the outer-sphere complex.
Orlistat forms a covalent but reversible bond with the active site serine residue of pancreatic lipase, rendering it unable to hydrolyze dietary fat into free fatty acids, therefore reducing the absorption of dietary fat [14].
Regarding compound 3s, the 2D and 3D ligand interaction simulations (Figs. 4 and 5) showed that 3s binds in the same fashion to the co-crystallized ligand displaying two hydrogen bonds with the active pocket amino acid Met 98 leading to an overall binding energy of = −11.64 kcal/mol (Table 3).
While substrates produce a fluorescent signal as a result of processing by a protease, ABPs are retained at the site of proteolysis due to formation of a permanent covalent bond with the active site catalytic residue.
As an alternative to substrates, fluorescent activity based probes have also been reported 3, 4. Unlike substrates, these reagents label target proteases through the formation of a covalent bond with the active site cysteine (Fig. 1B).
