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The transformants were mostly produced after the bombardment of sago embryogenic callus using 280 psi of helium pressure with a 6 cm distance of the gun to the target and with once (1x) or twice (2x) bombardment (Table 2).
Of the gametophytes co-bombarded with the 35S:irintCrChlI and pFF19G and having a colorless or GUS positive phenotype, ~89% were both colorless and GUS positive seven days after bombardment (Table 1), indicating a high efficiency of introduction of both plasmids into the same cell.
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At the same time, Hall Effect data simulation shows the composition reduction after Ag+ ion bombardment (see Table 1), which is agree with XRD results.
The UV/TMP method produced a much higher frequency than the bombardment protocol (Additional file 2, Table S1) [ 6].
About one-half of the colorless gametophytes that developed after bombardment were also GUS positive (Table 1), although the intensity of GUS staining was less than that observed in gametophytes bombarded with pFF19G (data not shown).
Sheltering in a rebel-controlled hospital in the bombed-out city, the men ate pasta as their table shook from artillery bombardment.
It's now a war of airstrikes, night raids and body counts that is supposed to force the Taliban to the negotiating table, similar to the aerial bombardment of North Vietnam that was designed to bring the Vietcong to their knees.
Particle bombardment produced the highest mean levels of luciferase activity (Table 1).
The most common events (Table 3) included mopping-up operations, aerial bombardment, mortar fire, attack on house or village, crossfire, burning of houses, and destruction of property.
About 60% of the hermaphrodites co-bombarded with the construct 35S:intantiCrUrod (Fig. 1) and pFF19G developed one or two discrete necrotic lesions within one week of bombardment (Figs. 4D,4E) and were also GUS positive (Table 1).
In this paper, particle bombardment immunization pushs T cells forward to CD4+ T cells (Table 2).
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