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Cotton bolls were collected at fibre initiation (−2, -1, 0, 1 and 2 dpa), elongation (5, 10 and 15 dpa) and secondary cell wall synthesis (20 dpa) stages.
Developing bolls were collected at 9 am at 3-day intervals from 13 through 25 DPA, and fibers were dissected from the cotton bolls immediately, frozen in liquid nitrogen and stored at −80°C.
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(They were collected at the end).
All data were collected at sunny middays.
Clinical data were collected at the National Institute for Infectious Disease LL.
This is identical to an analysis with individual plants as the experimental unit, as only one boll was collected from each sampled plant.
Smartphones are collected at the door.
Lentivirus supernatant was collected at 48 hours.
Flower buds at -3 DPA and flowers at 0 DPA were collected individually from the 60 plants in morning (9 00 11 00); the ovules were dissected from five bolls cottected in each of 60 plants, frozen in liquid nitrogen, and stored at -80°C until use.
At least 25000 events were collected.
Bolls were primarily collected from 12 00-4 00 pm to minimize potential expression variability associated with time of day.
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