Sentence examples for boiling steps from inspiring English sources

Cellular material was lysed and crude mixed-community genomic DNA prepared from each specimen by two 10-minute boiling steps followed by freezing at −20°C.

The heating or boiling steps required for the Schales' procedure or DNS method are not necessary, which renders the ChitO-based method extremely easy.

If you plan to follow through with the boiling steps, however, cut a vertically straight circle all the way around the core.

The main difference with the conventional Soxhlet is the incorporation of a previous step in which the sample is completely submerged in the extraction solvent in order to facilitate the rapid solubilization of analyte in boiling solvent (the boiling step); it allows a more efficient extraction and reduces the extraction time and solvent volume.

As a corroborative datum, a 10 min boiling step of the protein extract preceding the start of Hz formation assay reduced its ability to initiate Hz nucleation.

WT TTR and FT2·WT TTR in Laemmli sample buffer with or without a boiling step were analyzed in an 11% PAGE system.

One argument that lay at the heart of the original discussion was in reference to a boiling step in the purification protocol of bacterially expressed, recombinant α-synuclein.

It is important to note that the boiling step, an essential step in the Schales' procedure, is omitted from the ChitO assay, which represents one of the main advantages.

Histone samples, from both ChIP input and ChIP elutions, were prepared as described previously [ 27], with the exception that the acid extraction step was simply replaced by a boiling step for the ChIP elutions.

Lipid peroxidation was measured by the TBARSs (thiobarbituric acid-reactive substances) test [ 31], except 0.07 mM per assay of BHT (butylated hydroxytoluene) was added to prevent artificial lipid peroxidation during the boiling step [ 32].

The use of these oxidases in combination with HRP constitutes a fast and sensitive method to detect chitinase and cellulase activity, without the necessity of a boiling step, commonly employed in other methods.