Sentence examples for boiling of the samples from inspiring English sources
The reaction was stopped via boiling of the samples for 10 min. The organic acids and adenosine nucleotides were subsequently extracted for HPLC analysis.
The assay was terminated by the addition of 15 µl of 2×SDS sample buffer and boiling of the samples for 5 min. The sample was then subjected to SDS-PAGE and exposed to photographic film for autoradiography.
The boiling of the samples with chelating resin and subsequent DNA purification with silica columns seem to replace the need for cross-linking reversal and proteinase K treatment.
Samples of purified HSA (Sigma-Aldrich) were incubated with increasing concentrations of SG PBSS, 37°C, 20 hr) and subjected to SDS PAGE after reduction and boiling of the samples.
The WMS and PS of these volunteers was also used in a TGM assay to determine whether cross-linking of the saliva could be seen visually using gel electrophoresis run under non-reducing conditions (no DTT and no boiling of the samples).
It should be noted that the presence of a strong ALG-2 signal in the ALG-2.1 and ALG-2 pull-down lanes in Fig. 4 is due to recombinant ALG-2 eluted from the ALG-2 beads following boiling of the samples (as is also seen in Fig. 2) which served as a loading control.
In order to perform the zymogram analysis, the enzyme was electrophoresed as 12% SDS-PAGE without addition of beta-mercaptoethanol and without boiling of the sample.
For subsequent analysis by western blotting, proteins were eluted by boiling of the beads in Laemmli-SDS sample buffer.
After 15 minutes of boiling, the samples were smashed with glass beads (Mini-Beadbeater, Merlin Diagnostic Systems, Breda, The Netherlands) for two minutes.
In our study, when spiked into MBM, PrPsc was only detectable by Western Blot after boiling of sample in detergent.
