Sentence examples for boiling of samples from inspiring English sources

In some experiments, boiling of samples prior to SDS PAGE was avoided as specified.

Using freshly prepared sample buffer and boiling of samples for at least 10 min resulted in complete denaturation of the dimer into the monomer.

In our study, when spiked into MBM, PrPsc was only detectable by Western Blot after boiling of sample in detergent.

The reaction was stopped via boiling of the samples for 10 min. The organic acids and adenosine nucleotides were subsequently extracted for HPLC analysis.

In order to perform the zymogram analysis, the enzyme was electrophoresed as 12% SDS-PAGE without addition of beta-mercaptoethanol and without boiling of the sample.

Two wash steps with IP buffer preceded boiling of beads in denaturing sample buffer at 95°C for 5 min.

The assay was terminated by the addition of 15 µl of 2×SDS sample buffer and boiling of the samples for 5 min. The sample was then subjected to SDS-PAGE and exposed to photographic film for autoradiography.

After 15 minutes of boiling, the samples were smashed with glass beads (Mini-Beadbeater, Merlin Diagnostic Systems, Breda, The Netherlands) for two minutes.

An easy to implement sample preparation method was also investigated during this study, which was boiling blood samples of the patients for 10 minutes to release the parasite DNA.

Samples of purified HSA (Sigma-Aldrich) were incubated with increasing concentrations of SG PBSS, 37°C, 20 hr) and subjected to SDS PAGE after reduction and boiling of the samples.

The boiling of the samples with chelating resin and subsequent DNA purification with silica columns seem to replace the need for cross-linking reversal and proteinase K treatment.